Plasma Cancer Therapy by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9783030499662
Publisher: Springer International Publishing

Since cancer patients often receive drugs, Bekeschus and colleagues next asked the question whether there might be an additive or synergistic activity with oxidizing liquids and chemotherapy. After screening a drug library of 80 kinase-inhibitors, they found a number of targets showing promising synergistic activity in several human cancer cell lines assayed under 2D and 3D tumor spheroid conditions [56]. To extend this idea closer to the clinic, a subsequent investigation was concerned with using plasma-treated ringer’s lactate in combination with gemcitabine or cisplatin, two drugs applied in the treatment schemes of pancreatic cancer patients in the clinical setting [57]. The main findings were that additive toxicity was present in the combination treatment, leading to a decline in metabolic activity and cell growth, and an increase of apoptosis and cell cycle arrest. Importantly, the additive toxicity was found in both 2D cancer cell lines and 3D tumors grown in the in ovo model. Mechanistically, this study is important because it analyzed intracellular drug concentrations by mass spectrometry. Interestingly, drug concentrations were in the tendency higher in cells receiving drugs alone, while combination treatment with plasma-treated ringer’s lactate decreased the concentration (Fig. 6.13). This might be an indicator of accelerated cell death with the combination treatment.

Fig. 6.13Mass spectrometry analysis of intracellular chemotherapy drug concentrations (Gemcitabine, Cisplatin) upon exposure to plasma-treated Ringer’s Lactate solution during short-term and long-term culture in MiaPaca (a,b) and PatuS (c,d) human pancreatic cancer cells in vitro. (Reproduced from Liedtke et al. (2020) [57])

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